38.7 Folate:B12

38.7.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 33.9677450 1 <0.0001 ***
folate_B12 0.2922864 1 0.6 ns
Study 0.0030706 1 1.0 ns
GA_samp 20.0938411 1 <0.0001 ***
folate_B12:Study 0.9826171 1 0.3 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 6.6942890 1 0.010 *
folate_B12 4.6512349 1 0.03 *
Study 0.0693763 1 0.8 ns
GA_samp 323.6089803 1 <0.0001 ***
folate_B12:Study 4.1665896 1 0.04 *
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 24.247063 1 <0.0001 ***
folate_B12 3.087119 1 0.08 ns
Study 2.290189 1 0.1 ns
GA_samp 96.394483 1 <0.0001 ***
folate_B12:Study 2.838209 1 0.09 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 folate_B12 1 Inf 0.139 0.139 0.7 ns
3 Study 1 Inf 1.655 1.655 0.2 ns
4 GA_samp 1 Inf 0.991 0.991 0.3 ns
2 folate_B12:Study 1 Inf 0.164 0.164 0.7 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 folate_B12 1 Inf 0.363 0.363 0.5 ns
3 Study 1 Inf 3.897 3.897 0.05 ns
4 GA_samp 1 Inf 0.502 0.502 0.5 ns
2 folate_B12:Study 1 Inf 0.043 0.043 0.8 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 folate_B12 1 Inf 1.315 1.315 0.3 ns
3 Study 1 Inf 16.057 16.057 <0.0001 ***
4 GA_samp 1 Inf 0.185 0.185 0.7 ns
2 folate_B12:Study 1 Inf 0.265 0.265 0.6 ns

38.7.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 14.82 (5.93, 36.94) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.33
StudySTOP 1.12 (0.82, 1.55) 0.48
GA_samp 1.13 (1.07, 1.20) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.96 (1.16, 3.25) 0.01
folate_B12 1.00 (1.00, 1.00) 0.03
StudySTOP 1.03 (0.82, 1.30) 0.79
GA_samp 1.34 (1.29, 1.38) <0.0001
folate_B12:StudySTOP 1.00 (1.00, 1.00) 0.04
GH2 (ng/ml)
Var Effect P
(Intercept) 0.16 (0.09, 0.32) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.61
StudySTOP 0.93 (0.73, 1.19) 0.56
GA_samp 1.22 (1.17, 1.27) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 17.79 (4.81, 65.85) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.48
StudySTOP 0.65 (0.41, 1.03) 0.07
GA_samp 1.04 (0.96, 1.13) 0.32
HOMA-IR
Var Effect P
(Intercept) 5.50 (1.36, 22.29) 0.02
folate_B12 1.00 (1.00, 1.00) 0.37
StudySTOP 0.51 (0.31, 0.85) 0.009
GA_samp 1.03 (0.95, 1.13) 0.48
Glucose (mmol/L)
Var Effect P
(Intercept) 6.73 (5.15, 8.78) <0.0001
folate_B12 1.00 (1.00, 1.00) 0.21
StudySTOP 0.79 (0.73, 0.86) <0.0001
GA_samp 0.99 (0.97, 1.02) 0.65

38.7.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Folate:B12 ratio and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 71.60574 14.86257 52.74518 47.21980 108.5854
STOP 80.40635 13.27427 22.96752 57.14272 113.1409
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 62.09345 4.870901 111.51851 53.15462 72.53548
STOP 73.39619 3.059959 29.65228 67.40274 79.92256
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 1.888354 0.2043171 133.89526 1.524561 2.338957
STOP 1.755293 0.0921474 39.51082 1.578531 1.951848
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 28.59453 5.779733 Inf 19.24119 42.49462
STOP 18.55478 1.473926 Inf 15.87959 21.68065
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 7.759125 1.6807764 Inf 5.074883 11.863134
STOP 3.995353 0.3419368 Inf 3.378363 4.725025
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 6.134155 0.2493014 Inf 5.664487 6.642765
STOP 4.863797 0.1061501 Inf 4.660134 5.076361

38.7.4 Comparisons

Effect for every 100 unit increase in Folate:B12 ratio.
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) folate_B12241.85 / folate_B12141.85 SCOPE 1.02 (0.98, 1.07) 0.3
PRL (ng/ml) folate_B12241.85 / folate_B12141.85 STOP 1.02 (0.98, 1.07) 0.3
hPL (μg/ml) folate_B12241.85 / folate_B12141.85 SCOPE 0.93 (0.88, 0.99) 0.03
hPL (μg/ml) folate_B12241.85 / folate_B12141.85 STOP 1.00 (0.97, 1.03) 0.9
GH2 (ng/ml) folate_B12241.85 / folate_B12141.85 SCOPE 0.99 (0.96, 1.03) 0.6
GH2 (ng/ml) folate_B12241.85 / folate_B12141.85 STOP 0.99 (0.96, 1.03) 0.6
Insulin (uU/ml) folate_B12241.85 / folate_B12141.85 SCOPE 0.98 (0.94, 1.03) 0.5
Insulin (uU/ml) folate_B12241.85 / folate_B12141.85 STOP 0.98 (0.94, 1.03) 0.5
HOMA-IR folate_B12241.85 / folate_B12141.85 SCOPE 0.98 (0.92, 1.03) 0.4
HOMA-IR folate_B12241.85 / folate_B12141.85 STOP 0.98 (0.92, 1.03) 0.4
Glucose (mmol/L) folate_B12241.85 / folate_B12141.85 SCOPE 0.99 (0.97, 1.01) 0.2
Glucose (mmol/L) folate_B12241.85 / folate_B12141.85 STOP 0.99 (0.97, 1.01) 0.2

38.7.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

38.7.6 Plots